High Performance Liquid Chromatography (HPLC)
Separating substances by how fast they flow in a liquid stream.
The HPLC instrument and operator Britt Sommer.
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Comparing two HPLC chromatograms.
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The technique: The High Performance Liquid Chromatography (HPLC) is used widely as a routine analysis method. The demands of high accuracy, sensitivity and reproducibility are of vital importance. Chromatography now embraces a variety of processes which are based on differential distributions of the sample components between two phases. One phase remains fixed in the system and is called the stationary phase. The other phase, called the mobile phase, percolates through the interstices, or over the surface, of the fixed phase. The movement of the mobile phase causes a differential migration of the sample components. A given sample molecule finds itself either in the stationary phase, in which case it is making no forward progress, or in the mobile phase, in which case it is being carried along the column at the same velocity as the mobile phase. The mobile phase acts as a carrier, it provides the transportation. The stationary phase acts as a retarder. Liquid chromatography is particularly useful for samples containing large molecules. It is an important tool for analysis of e.g additives in polymers.
The instrument: Our system is from Perkin Elmer, and includes the following modules:
- Series 200 LC gradient pump
- Series 200 Autosampler
- Series 200 Diodes Array detector
Sample requirements:
Amount | > 3 mg |
Solubility | in water or organic solvents |
Contact persons:
Ferdinand Männle, phone: +47 98 28 24 91
Britt Sommer, phone: +47 98 28 39 39